Abstract: Series 110, Lecture 7
The Harvey Lectures Series 110 (2014—2015)
Lecture #7: Thursday, May 21, 2015 — Watch Video of Lecture
Imaging Life at High Spatiotemporal Resolution
Eric Betzig, PhD
Group Leader
Janelia Research Campus, Howard Hughes Medical Institute
Howard Hughes Medical Institute, Janelia Research Campus
Ashburn, Virginia
As our understanding of biological systems as increased, so has the complexity of our questions and the need for more advanced optical tools to answer them. For example, there is a hundred-fold gap between the resolution of conventional optical microscopy and the scale at which molecules self-assemble to form sub-cellular structures. Furthermore, as we attempt to peer more closely at the dynamic complexity of living systems, the actinic glare of our microscopes can adversely influence the specimens we hope to study. Finally, the heterogeneity of living tissue can seriously impede our ability to image at high resolution, due to the resulting warping and scattering of light rays. I will describe three areas focused on addressing these challenges: super-resolution microscopy for imaging specific proteins within cells down to near-molecular resolution; plane illumination microscopy using non-diffracting beams for noninvasive imaging of three-dimensional dynamics within live cells and embryos; and adaptive optics to recover optimal images from within optically heterogeneous specimens.